An experimental study on early pathogenesis of a very mvirulent isolate of infectious bursal disease virus, employing immunohistochemistry
نویسندگان
چکیده مقاله:
In this study, immunohistochemistry was used to clarify the early stages of viral kinetics and cycliccourse of IBDV, IR499, which has been described earlier as a very virulent strain (vvIBDV). Fifteen, 4-week-old SPF chickens were inoculated with 10³ EID50 of vvIBDV, IR499, via oculo/nasal route. Five birdsserved as controls, and inoculated with phosphate buffered saline (PBS). The birds were then bled, and tissue samples from bursa of Fabricius, cecal tonsils, liver, spleen, thymus and thigh muscle were harvested at 3, 6, 12, 24 and 48 h post-inoculation (p.i.). Typical positive signals were first observed as early as 3 h p.i. in lymphoid cells of cecal tonsils (the organ of primary affinity) and Kupfer cells of liver. Viral antigens inbursa were first found at 6 h p.i. which represents the occurrence of primary viraemia. After secondaryviraemia, the virus appeared in spleen and thymus at 12 h p.i. These findings at early stages of viral infection, represented IBDV, IR499, as a very virulent strain with a rapid and generalized course, at in vivo level.
منابع مشابه
an experimental study on early pathogenesis of a very mvirulent isolate of infectious bursal disease virus, employing immunohistochemistry
in this study, immunohistochemistry was used to clarify the early stages of viral kinetics and cycliccourse of ibdv, ir499, which has been described earlier as a very virulent strain (vvibdv). fifteen, 4-week-old spf chickens were inoculated with 10³ eid50 of vvibdv, ir499, via oculo/nasal route. five birdsserved as controls, and inoculated with phosphate buffered saline (pbs). the birds were t...
متن کاملEvaluation of Experimental Inactivated Infectious Bursal Disease Vaccine Using a Local Very Virulent Pathotype Strain
متن کامل
Vaccination of Chicks with an Experimental Oil-emulsion Vaccine against Infectious Bursal Disease Virus
متن کامل
Very Virulent Infectious Bursal Disease Virus
The primary feature of vvIBDV is the ability to induce high mortality. Mortality ranges from 5% – 25% in broilers and 30% – 70% in layers. Surviving birds are severely affected with a high condemnation rate. vvIBDV induces clinical signs similar to Gumboro disease, which is induced by classical virulent strains of IBDV, except that the disease is more pronounced and acute in individual birds an...
متن کاملEffect of experimental infectious bursal disease virus on clinical signs and pathogenesis of avian influenza virus H9N2 in turkey by real time PCR
Infectious bursal disease virus (IBDV) in turkeys may result in immunosuppression, and inability of turkeys to resist nonpathogenic or less pathogenic organisms. A total number of 120 day-old commercial male turkeys were purchased and blood samples were collected from 20 day-old turkeys, remaining 100 were divided into four equal groups and kept in separated rooms. Groups 1 and 2 were infected ...
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ذخیره در منابع من قبلا به منابع من ذحیره شده{@ msg_add @}
عنوان ژورنال
دوره 10 شماره 2
صفحات 125- 131
تاریخ انتشار 2009-06-20
با دنبال کردن یک ژورنال هنگامی که شماره جدید این ژورنال منتشر می شود به شما از طریق ایمیل اطلاع داده می شود.
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023